Drosophila gamma-aminobutyric acid receptor gene Rdl shows extensive alternative splicing

J Neurochem. 1993 Jun;60(6):2323-6. doi: 10.1111/j.1471-4159.1993.tb03523.x.


The Drosophila gamma-aminobutyric acid (GABA) receptor subunit gene Rdl was isolated on the basis of a mutant phenotype showing high levels of insensitivity to picrotoxinin and cyclodiene insecticides. Following analysis of two dissimilar cDNAs isolated from the locus, we report that Rdl undergoes extensive alternative splicing at two locations in the putative extracellular domain. At each location a choice is made between exons of the same size: "a" or "b" (23 amino acids long with two substitutions) and "c" or "d" (46 residues long with 10 substitutions). The function of these alternative exons remains unclear; however, exon d contains a putative site for casein kinase II phosphorylation. All possible combinations of exons (a with c or d and b with c or d) were found in RNA isolated from early embryos. This is the first demonstration of alternative splicing in a GABA receptor gene from invertebrates.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alternative Splicing*
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • DNA / genetics
  • DNA / isolation & purification
  • Drosophila melanogaster / genetics*
  • Drosophila melanogaster / metabolism
  • Exons
  • Gene Library
  • Molecular Sequence Data
  • Oligodeoxyribonucleotides
  • Poly A / genetics
  • Poly A / isolation & purification
  • Polymerase Chain Reaction / methods
  • RNA / genetics
  • RNA / isolation & purification
  • RNA, Messenger
  • Receptors, GABA-A / genetics*
  • Restriction Mapping
  • Sequence Homology, Nucleic Acid


  • Oligodeoxyribonucleotides
  • RNA, Messenger
  • Receptors, GABA-A
  • Poly A
  • RNA
  • DNA