Identification of peptide sequences that potentially trigger HLA-A2.1-restricted cytotoxic T lymphocytes

Eur J Immunol. 1993 Jun;23(6):1215-9. doi: 10.1002/eji.1830230603.


We used the human processing defective cell line 174CEM.T2 (T2) to identify potential cytotoxic T lymphocyte (CTL) epitopes of human proteins. Exogenously added peptides can increase the number of properly folded HLA-A2.1 molecules on the cell surface of T2 cells, as shown by immunofluorescence measurements using the mouse monoclonal antibody BB7.2 (anti-HLA-A2.1) and fluorescein isothiocyanate-labeled goat anti-mouse F(ab')2 antibody. The peptides were selected on the basis of a computer score derived from the recently described HLA-A2.1 specific motif. Analysis of the influenza matrix protein showed that 15 out of 35 high-scoring peptides up-regulate the expression of HLA-A2.1 molecules on the T2 cell surface. The combination of the computer scoring program and an immunofluorescence-based peptide binding assay allows rapid detection of potential CTL target peptides.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Antigens, Viral / immunology*
  • Cytotoxicity, Immunologic*
  • Epitopes
  • HLA-A2 Antigen / immunology*
  • Humans
  • Immunity, Cellular
  • In Vitro Techniques
  • Influenza A virus / immunology
  • Molecular Sequence Data
  • Peptides / immunology*
  • Structure-Activity Relationship
  • T-Lymphocytes, Cytotoxic / immunology*
  • Viral Matrix Proteins / chemistry
  • Viral Matrix Proteins / immunology*
  • beta 2-Microglobulin / immunology


  • Antigens, Viral
  • Epitopes
  • HLA-A2 Antigen
  • M-protein, influenza virus
  • Peptides
  • Viral Matrix Proteins
  • beta 2-Microglobulin