Abstract
Alignment of previously identified HLA B8-restricted cytotoxic T-cell (CTL) epitopes revealed a common motif: x, x, K/R, x, R/K, x, W/Y, x, I/L. Of five motif-conforming peptides tested, ELRSRYWAI was found to represent a new epitope from influenza nucleoprotein (NP). High concentrations of some peptides gave cross-reactive stimulation illustrating the requirement to confirm epitope assignment with recombinant vaccinias and highlighting a potential problem of peptide-based CTL vaccines.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Antigens, Viral / analysis*
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Dose-Response Relationship, Immunologic
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Epitopes / analysis*
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HLA-B8 Antigen / analysis*
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Humans
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Molecular Sequence Data
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Nucleocapsid Proteins
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Nucleoproteins / immunology*
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RNA-Binding Proteins*
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Sequence Homology, Amino Acid
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T-Lymphocytes, Cytotoxic / immunology*
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Viral Core Proteins / immunology*
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Viral Vaccines / immunology
Substances
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Antigens, Viral
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Epitopes
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HLA-B8 Antigen
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NP protein, Influenza A virus
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Nucleocapsid Proteins
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Nucleoproteins
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RNA-Binding Proteins
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Viral Core Proteins
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Viral Vaccines