Morphological observations on pancreatic islet blood vessels in low-dose streptozocin-treated mice

J Anat. 1993 Feb;182 ( Pt 1)(Pt 1):45-53.


The aim of this study was to observe modifications occurring within the blood vessels of pancreatic islets in low-dose streptozocin (STZ)-treated mice. Forty-two male C57BL/6J mice were given 40 mg STZ on 5 consecutive days and killed 6 or 10 d after the first STZ injection. Immunocytochemistry revealed a dense accumulation of cells carrying the 7D10 epitope, related to macrophage inhibitor factor, arranged around the 'vascular pole' of the islets on d 6, and a small number of positive cells within the islets on d 10. Many Ia positive cells (equivalent to MHC class II antigen positive elements) were also found at the periphery of the islet. Ultrastructural observations showed the presence of monocytes within almost all the capillary vessels encircling the islets and entering them at the 'vascular pole' (d 6). On d 10 capillary lumina no longer contained marginating monocytes but activated macrophages were seen in the islet parenchyma.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blood Vessels / ultrastructure
  • Diabetes Mellitus, Experimental / pathology*
  • Epitopes / analysis
  • Histocompatibility Antigens Class II / analysis
  • Islets of Langerhans / blood supply*
  • Islets of Langerhans / drug effects
  • Islets of Langerhans / immunology
  • Macrophages / ultrastructure
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Microscopy, Electron
  • Monocytes / ultrastructure
  • Time Factors


  • Epitopes
  • Histocompatibility Antigens Class II