Detection of a circulating form of vascular cell adhesion molecule-1: raised levels in rheumatoid arthritis and systemic lupus erythematosus

Clin Exp Immunol. 1993 Jun;92(3):412-8. doi: 10.1111/j.1365-2249.1993.tb03413.x.


We have developed a panel of MoAbs against four separate but overlapping epitopes on endothelial cell (EC) vascular cell adhesion molecule-1 (VCAM-1). Two of the MoAbs (1G11 and 1E5) inhibited T cell adhesion to tumour necrosis factor (TNF)-activated EC, whilst two MoAbs (1.4C3 and 6D9) did not. Using these MoAbs we have identified a circulating form of VCAM-1 (cVCAM-1) which has identical epitope distribution to the EC form, and which is able to support the adhesion of the human lymphoblastoid cell line Jurkat J6 by a VLA-4- and VCAM-1-dependent mechanism when immobilized from plasma. cVCAM-1 isolated by immunoaffinity and size-exclusion chromatographies was shown by SDS-PAGE to have an apparent mol. wt of 85-90 kD. Levels of cVCAM-1 were significantly raised (P < 0.001) in rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE) compared with normal individuals. It is possible that cVCAM-1 may be a useful plasma marker for the diagnosis and management of patients with inflammatory diseases. Furthermore, detection of elevated cVCAM-1 levels may act as a guide to the importance of VCAM-1-dependent cell adhesion in different pathological settings.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Antibodies, Monoclonal / immunology
  • Arthritis, Rheumatoid / blood
  • Arthritis, Rheumatoid / immunology*
  • Cell Adhesion
  • Cell Adhesion Molecules / blood*
  • Cells, Cultured
  • Electrophoresis, Polyacrylamide Gel
  • Endothelium, Vascular / immunology
  • Enzyme-Linked Immunosorbent Assay
  • Epitopes / immunology
  • Female
  • Humans
  • Lupus Erythematosus, Systemic / blood
  • Lupus Erythematosus, Systemic / immunology*
  • Male
  • Middle Aged
  • T-Lymphocytes / immunology
  • Vascular Cell Adhesion Molecule-1


  • Antibodies, Monoclonal
  • Cell Adhesion Molecules
  • Epitopes
  • Vascular Cell Adhesion Molecule-1