Epitope specificity of H-2Kb-restricted, HSV-1-, and HSV-2-cross-reactive cytotoxic T lymphocyte clones

Virology. 1993 Jul;195(1):62-70. doi: 10.1006/viro.1993.1346.

Abstract

HSV-1-specific and HSV-1/HSV-2-cross-reactive H-2Kb-restricted cytotoxic T lymphocyte (CTL) clones were derived from a pool of splenic memory CTL (CTLm) obtained from HSV-1-infected C57BL/6 mice. Two of the HSV-1/HSV-2-cross-reactive CTL clones recognized HSV gB since H-2b cells infected with a recombinant adenovirus vector expressing HSV glycoprotein B (gB) provided a target for these CTL clones. The CTL recognition epitope was precisely defined as HSV-1 gB residues 498-505 using synthetic peptides and conforms to a predicted H-2Kb-binding motif. Immunization of C57BL/6 mice with the free synthetic peptide corresponding to this predicted minimal epitope (HSV-1 gB498-505) resulted in the generation of HSV-gB epitope-specific CD8+ CTL in the popliteal lymph nodes. The peptide-induced CTL recognize and lyse HSV-1 infected H-2b cells or cells pulsed with the synthetic peptide, gB498-505. The availability of CTL clones directed to this predicted minimal HSV CTL epitope should be helpful in understanding processing of HSV glycoprotein B and presentation of this CTL recognition epitope.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • CD8 Antigens / immunology
  • Cell Line
  • Clone Cells
  • Epitopes / immunology*
  • Flow Cytometry
  • H-2 Antigens / immunology*
  • Immunologic Memory
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • Phenotype
  • Simplexvirus / immunology*
  • T-Lymphocytes, Cytotoxic / immunology*
  • Viral Envelope Proteins / immunology*

Substances

  • CD8 Antigens
  • Epitopes
  • H-2 Antigens
  • H-2Kb protein, mouse
  • Viral Envelope Proteins
  • glycoprotein B, Simplexvirus