Different signals mediate transforming growth factor-beta 1-induced growth inhibition and extracellular matrix production in prostatic carcinoma cells

Exp Cell Res. 1993 Jul;207(1):1-7. doi: 10.1006/excr.1993.1156.


The effects of transforming growth factor-beta 1 (TGF-beta 1) on a human prostatic carcinoma cell line PC-3, and its subclone PC-3U, were investigated. Dose-dependent inhibition of [3H]thymidine incorporation in PC-3U cells was observed by addition of TGF-beta 1, although only 50% inhibition was obtained by high concentrations (12 nM) of TGF-beta 1. The growth inhibitory effects of TGF-beta 1 on PC-3 cells was insignificant. When 0.3 ng/ml of phorbol 12-myristate 13-acetate (PMA) was added together with TGF-beta 1, TGF-beta 1 inhibited growth of PC-3 cells (about 50% inhibition), and the growth inhibitory activity of TGF-beta 1 in PC-3U cells was enhanced (more than 90% inhibition). Affinity crosslinking studies revealed that both cell lines possess all of the three described forms of TGF-beta receptors. The intensities of the crosslinked bands were weaker in the PC-3 cells than in PC-3U cells, and those were not increased by the addition of PMA. The expression of the TGF-beta type II receptor mRNA did not change after the addition of PMA or TGF-beta 1. These results suggest that the effects of PMA involved downstream components of the signal transduction pathway of TGF-beta 1. TGF-beta 1 is known to stimulate the production of extracellular matrix proteins and to induce changes in the expression of nuclear transcription factor genes. In both PC-3 and PC-3U cells, TGF-beta 1 was found to stimulate the induction of fibronectin and plasminogen activator inhibitor-1 and the expression of junB mRNA, and PMA did not affect these responses. Thus, PC-3 and PC-3U cells, which are partially resistant to the growth inhibitory activity of TGF-beta 1, could still respond to TGF-beta 1 by extracellular matrix production, independent of PMA action. These results suggest that different signalling pathways mediate TGF-beta 1-induced growth inhibition and stimulation of extracellular matrix accumulation in these cells.

MeSH terms

  • Cell Division / drug effects
  • Clone Cells / drug effects*
  • Drug Resistance
  • Fibronectins / biosynthesis*
  • Fibrosis / physiopathology
  • Genes, jun / drug effects
  • Humans
  • Male
  • Plasminogen Activator Inhibitor 1 / biosynthesis
  • Prostatic Neoplasms / physiopathology
  • RNA / analysis
  • Receptors, Cell Surface / analysis
  • Receptors, Transforming Growth Factor beta
  • Signal Transduction*
  • Tetradecanoylphorbol Acetate / pharmacology
  • Thymidine / metabolism
  • Transforming Growth Factor beta / pharmacology*
  • Tumor Cells, Cultured / drug effects*


  • Fibronectins
  • Plasminogen Activator Inhibitor 1
  • Receptors, Cell Surface
  • Receptors, Transforming Growth Factor beta
  • Transforming Growth Factor beta
  • RNA
  • Tetradecanoylphorbol Acetate
  • Thymidine