Structural analysis of HLA-B40 epitopes

Hum Immunol. 1993 Mar;36(3):193-8. doi: 10.1016/0198-8859(93)90125-k.

Abstract

Two genes encoding HLA-B60 or HLA-B61 were cloned from Japanese and the exons of their genes were sequenced. One silent mutation was observed at the exon 1 between HLA-B60 (B*40012) and B*40011. Seven nucleotide substitutions were seen at the exon 3 between HLA-B61 (B*4006) and B*4002. Three substitutions at codon 95, CTC in B*4002 to TGG in B*4006, changed Leu in B*4002 to Trp in B*4006, while two substitutions at codon 97, AGC in B*4002 and ACG in B*4006, changed Ser in B*4002 to Thr in B*4006. Since B*4002 shares the epitope of alloantibodies specific for HLA-B61, two HLA-B61 subtypes are discriminated by two amino acid substitutions at residues 95 and 97. B*40012 and B*4006 differ by four amino acid substitutions on the beta sheet and five amino acid substitutions on the alpha 2 helix. Since the residues at the beta sheet seem hardly to affect the binding of alloantibody, it is suspected that the residues on the alpha 2 helix provide epitopes for alloantibodies that discriminate allospecificity between HLA-B60 and HLA-B61.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • Cytotoxicity Tests, Immunologic
  • DNA Mutational Analysis
  • Epitopes / chemistry
  • Epitopes / immunology
  • Genes, MHC Class II*
  • HLA-B Antigens / chemistry*
  • HLA-B Antigens / genetics
  • HLA-B Antigens / immunology
  • HLA-B40 Antigen
  • Humans
  • Models, Molecular
  • Molecular Sequence Data
  • Protein Conformation
  • Sequence Homology

Substances

  • Epitopes
  • HLA-B Antigens
  • HLA-B40 Antigen
  • HLA-B61 antigen

Associated data

  • GENBANK/M95530
  • GENBANK/M95531