Vitamin D receptor gene expression is up-regulated by 1, 25-dihydroxyvitamin D3 in 3T3-L1 preadipocytes

Biochem Biophys Res Commun. 1993 Jun 30;193(3):948-55. doi: 10.1006/bbrc.1993.1717.

Abstract

Vitamin D3, especially 1, 25-dihydroxyvitamin D3 (1, 25 (OH)2 D3), has been shown to inhibit the differentiation of preadipose cells. In the present study, the expression of vitamin D receptor (VDR) was examined by Northern blot analysis in rat adipose tissue and mouse 3T3-L1 adipose cells. The adipose tissue and 3T3-L1 cells expressed mRNA for VDR. Treatment of 3T3-L1 cells with 1 microM 1, 25 (OH)2 D3 led to a 4-5 fold increase in the VDR mRNA level. VDR mRNA expression was rapidly (within 4 hr) induced by physiological concentrations of 1,25 (OH)2 D3 in a dose-dependent manner. The induction of VDR expression did not require de novo protein synthesis but was completely abolished by an inhibitor of RNA synthesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3 Cells
  • Adipose Tissue / drug effects
  • Adipose Tissue / metabolism*
  • Animals
  • Blotting, Northern
  • Calcitriol / pharmacology*
  • Cycloheximide / pharmacology
  • Dactinomycin / pharmacology
  • Dose-Response Relationship, Drug
  • Gene Expression / drug effects*
  • Mice
  • Poly A / isolation & purification
  • Poly A / metabolism
  • RNA / isolation & purification
  • RNA / metabolism
  • RNA, Messenger / biosynthesis*
  • Rats
  • Receptors, Calcitriol
  • Receptors, Steroid / biosynthesis*
  • Receptors, Steroid / drug effects
  • Up-Regulation / drug effects

Substances

  • RNA, Messenger
  • Receptors, Calcitriol
  • Receptors, Steroid
  • Dactinomycin
  • Poly A
  • RNA
  • Cycloheximide
  • Calcitriol