Chemotaxins inhibit neutrophil adherence to and transmigration across cytokine-activated endothelium: correlation to the expression of L-selectin

Eur J Immunol. 1993 Jul;23(7):1481-7. doi: 10.1002/eji.1830230713.

Abstract

Non-activated neutrophils strongly adhere to cytokine-activated human umbilical vein endothelial cells (HUVE). However, activation of neutrophils by different chemotactic mediators led to potent inhibition of this endothelial-dependent interaction. For different formylated peptides, concentrations leading to maximal adherence inhibition coincided with those known for inducing maximal chemotactic migration of neutrophils. In terms of maximal adherence inhibition, a rank list was found in the order of N-formyl-Met-Leu-Phe > C5adesArg > interleukin-8 > C5a > or = leukotriene B4, whereas platelet-activating factor, and lipopolysaccharide showed no inhibition. This rank order was congruent to that of down-regulation of neutrophil L-selectin detected by the monoclonal antibody Leu-8. Moreover, the dose-dependent increase of neutrophil adherence inhibition corresponded to the loss of L-selectin expression. Concentrations higher than that required for maximal inhibition led to a dose-dependent decrease of inhibition, which was accompanied by increasing expression of neutrophil CD11/CD18. In contrast to the capacity of non-activated neutrophils to migrate across interleukin-1-activated HUVE monolayers, transmigration was significantly impaired after chemotactic activation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, CD / metabolism
  • CD11 Antigens
  • CD18 Antigens
  • Cell Adhesion
  • Cell Adhesion Molecules / metabolism*
  • Cells, Cultured
  • Chemotactic Factors / pharmacology*
  • Chemotaxis, Leukocyte*
  • Cytokines / pharmacology*
  • Endothelium, Vascular / cytology*
  • Humans
  • In Vitro Techniques
  • Interleukin-1 / pharmacology
  • L-Selectin
  • Lipopolysaccharides / pharmacology
  • N-Formylmethionine Leucyl-Phenylalanine / pharmacology
  • Neutrophils / cytology*
  • Tumor Necrosis Factor-alpha / pharmacology

Substances

  • Antigens, CD
  • CD11 Antigens
  • CD18 Antigens
  • Cell Adhesion Molecules
  • Chemotactic Factors
  • Cytokines
  • Interleukin-1
  • Lipopolysaccharides
  • Tumor Necrosis Factor-alpha
  • L-Selectin
  • N-Formylmethionine Leucyl-Phenylalanine