Further investigations on the expression of HLA-DR, CD33 and CD13 surface antigens in purified bone marrow and peripheral blood CD34+ haematopoietic progenitor cells

Br J Haematol. 1993 May;84(1):24-30. doi: 10.1111/j.1365-2141.1993.tb03021.x.


We evaluated the HLA-DR, CD33 and CD13 antigen expression on CD34+ haematopoietic progenitor cells (HPC) isolated from the bone marrow (BM) and peripheral blood (PB) of normal donors. The majority of both BM and PB CD34+ HPC expressed CD13 and HLA-DR. The coexpression of CD34 and CD33 was found in a minor CD34+ subset. After 7 d of culture in the presence of interleukin-3 and granulocyte-macrophage colony-stimulating factor, CD33 expression was detected in about 50% of HPC. At this point CD34 antigen expression was lost and CD13 and HLA-DR expression was partially lost. After 14 d of culture, the majority of HPC were CD33+. HPC maintained the capacity to generate colony forming unit granulocyte-macrophage but they lost the capacity to generate burst forming unit-erythroid. A correlation was found between the percentage of CD34+/HLA-DR+ cells and the total number of colony forming cells in unfractionated samples from BM and PB of patients with malignancies. These studies demonstrate that, in normal conditions, only a minor subset of CD34+ cells coexpress CD33 antigen either in BM or in PB and CD33 antigen is a lineage marker which is coexpressed with HLA-DR and CD13 on a progenitor committed to the granulocytic-macrophagic lineage.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, CD / analysis*
  • Antigens, CD34
  • Antigens, Differentiation, Myelomonocytic / analysis*
  • Antigens, Neoplasm / analysis
  • Bone Marrow / immunology
  • CD13 Antigens
  • Cell Differentiation / immunology
  • Cell Division / immunology
  • Cells, Cultured
  • Granulocyte-Macrophage Colony-Stimulating Factor / immunology
  • HLA-DR Antigens / analysis*
  • Hematopoietic Stem Cells / immunology*
  • Humans
  • Interleukin-3 / immunology
  • Sialic Acid Binding Ig-like Lectin 3


  • Antigens, CD
  • Antigens, CD34
  • Antigens, Differentiation, Myelomonocytic
  • Antigens, Neoplasm
  • CD33 protein, human
  • HLA-DR Antigens
  • Interleukin-3
  • Sialic Acid Binding Ig-like Lectin 3
  • Granulocyte-Macrophage Colony-Stimulating Factor
  • CD13 Antigens