Identification of O-phosphoamino acids in urine hydrolysate by gas chromatography-mass spectrometry

J Chromatogr. 1993 May 19;615(1):136-41. doi: 10.1016/0378-4347(93)80299-j.

Abstract

The occurrence of O-phosphoserine (P-Ser), O-phosphothreonine (P-Thr) and O-phosphotyrosine (P-Tyr) in the hydrolysate of human urine was demonstrated. Urine samples were separated into non-adsorbed and adsorbed fractions by DEAE-cellulose column chromatography. After acid and base hydrolyses of these fractions, the O-phosphoamino acids in the hydrolysates were converted into their N-isobutoxycarbonyl methyl ester derivatives and identified by gas chromatography-mass spectrometry. By gas chromatography with flame photometric detection, the urinary contents of P-Ser, P-Thr and P-Tyr were estimated to be 945 +/- 3, 109 +/- 3 and 2.9 +/- 0.2 ng/ml, respectively.

MeSH terms

  • Amino Acids / urine*
  • Chromatography, DEAE-Cellulose
  • Chromatography, Gas
  • Gas Chromatography-Mass Spectrometry
  • Humans
  • Hydrolysis
  • Phosphoserine / urine
  • Phosphothreonine / urine
  • Phosphotyrosine
  • Photometry
  • Tyrosine / analogs & derivatives
  • Tyrosine / urine

Substances

  • Amino Acids
  • Phosphothreonine
  • Phosphoserine
  • Phosphotyrosine
  • Tyrosine