A role for sialyl Lewis-X/A glycoconjugates in capillary morphogenesis

Nature. 1993 Sep 16;365(6443):267-9. doi: 10.1038/365267a0.


To identify cell adhesion molecules required for angiogenesis, we used an in vitro model in which bovine capillary endothelial cells can be induced to form capillary-like tubes. Monoclonal antibodies directed against the carbohydrate epitopes sialyl Lewis-X and sialyl Lewis-A inhibited capillary formation. We postulated that a member of the selectin family of adhesion molecules may be involved in capillary formation because these proteins bind to sialyl Lewis-X/A-containing ligands. We isolated a 2.8-kilobase complementary DNA from a bovine capillary endothelial cell cDNA library which encodes a polypeptide with 71% identity to human E-selectin. We report here that antibody directed against the bovine E-selectin inhibited capillary formation, suggesting that in addition to its role in leukocyte adhesion to endothelium, a form of E-selectin is involved in capillary morphogenesis.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Blotting, Northern
  • Capillaries / cytology*
  • Carbohydrate Sequence
  • Cattle
  • Cell Adhesion Molecules / physiology*
  • E-Selectin
  • Endothelium, Vascular / cytology
  • Glycoconjugates / physiology*
  • Humans
  • Immunologic Techniques
  • In Vitro Techniques
  • Lewis Blood Group Antigens / physiology*
  • Molecular Sequence Data
  • RNA, Messenger / analysis
  • Sequence Homology, Amino Acid


  • Cell Adhesion Molecules
  • E-Selectin
  • Glycoconjugates
  • Lewis Blood Group Antigens
  • RNA, Messenger

Associated data

  • GENBANK/L12039