Abstract
Sialyl-Lewisx (NeuAc alpha 2-->3Gal beta 1-->4[Fuc alpha 1-->3]GlcNAc] has been identified as a ligand for E-selectin, P-selectin and recently also for L-selectin. We have synthesized the sialyl-Lewisx tetrasaccharide by total enzymatic synthesis from N-acetyllactosamine using a placental alpha 2-->3-sialyltransferase specific for type-2 chain acceptors, followed by a cloned human alpha 1-->3-fucosyltransferase (FucTV, the 'plasma-type' enzyme). This procedure resulted in the tetrasaccharide in a 61% overall yield.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Sugars / metabolism
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Carbohydrate Sequence
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Cell Adhesion
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Cell Adhesion Molecules / metabolism*
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Cells, Cultured
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E-Selectin
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Fucosyltransferases / metabolism
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Humans
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Lewis X Antigen / chemistry*
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Lewis X Antigen / metabolism
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Molecular Sequence Data
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Oligosaccharides / chemical synthesis*
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Oligosaccharides / metabolism
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Sialyltransferases / metabolism
Substances
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Amino Sugars
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Cell Adhesion Molecules
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E-Selectin
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Lewis X Antigen
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Oligosaccharides
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N-acetyllactosamine
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Fucosyltransferases
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Sialyltransferases