A comparative evaluation of the metabolic profiles of normal and inflammatory knee-joint synovial fluids by high resolution proton NMR spectroscopy

FEBS Lett. 1993 Oct 18;332(3):221-5. doi: 10.1016/0014-5793(93)80636-9.


High resolution 1H NMR spectroscopy has been employed to investigate the metabolic profile of healthy human knee-joint synovial fluid (SF) and the biochemical data acquired have been compared with those of matched serum, and inflammatory knee-joint SF samples. Results obtained indicate that the healthy human knee-joint has a hypoxic status (high lactate level when expressed relative to that of paired serum) that is milder than that of the inflamed human knee-joint. Moreover, normal SF differs from that of inflammatory SF in that it contains little or no NMR-detectable lipoprotein-associated fatty acids and 'acute-phase' glycoproteins, an observation reflecting the limited passage of these macromolecules from plasma into the synovial space in healthy subjects.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acute-Phase Proteins / metabolism
  • Adult
  • Aged
  • Arthritis, Rheumatoid / blood
  • Arthritis, Rheumatoid / metabolism*
  • Fatty Acids / metabolism
  • Humans
  • Inflammation
  • Knee Joint*
  • Lactates / blood
  • Lactates / metabolism
  • Lipoproteins / metabolism
  • Magnetic Resonance Spectroscopy / methods
  • Male
  • Middle Aged
  • Reference Values
  • Synovial Fluid / metabolism*


  • Acute-Phase Proteins
  • Fatty Acids
  • Lactates
  • Lipoproteins