Species of Borrelia distinguished by restriction site polymorphisms in 16S rRNA genes

FEMS Microbiol Lett. 1993 Aug 1;111(2-3):239-43. doi: 10.1111/j.1574-6968.1993.tb06392.x.

Abstract

Three phyletic groups of Borrelia associated with Lyme disease, B. burgdorferi, B. garinii and group VS461 can be distinguished from each other and other species of Borrelia by BfaI restriction site polymorphisms in PCR amplified 16S rRNA genes. One strain isolated from an Ixodes pacificus tick in California that was previously unclassifiable was distinguishable from B. burgdorferi by an Mn/I restriction site polymorphism.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Borrelia / classification*
  • Borrelia / genetics*
  • Borrelia burgdorferi Group / classification
  • Borrelia burgdorferi Group / genetics
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / genetics
  • Genes, Bacterial
  • Molecular Sequence Data
  • Polymorphism, Restriction Fragment Length
  • RNA, Bacterial / genetics*
  • RNA, Ribosomal, 16S / genetics*
  • Species Specificity

Substances

  • DNA, Bacterial
  • DNA, Ribosomal
  • RNA, Bacterial
  • RNA, Ribosomal, 16S

Associated data

  • GENBANK/M60967
  • GENBANK/M60968
  • GENBANK/M60969
  • GENBANK/M64309
  • GENBANK/M64310
  • GENBANK/M64311
  • GENBANK/M64312
  • GENBANK/M72397
  • GENBANK/M72398
  • GENBANK/X57404