Dissimilatory sulphite reductase from Archaeoglobus fulgidus: physico-chemical properties of the enzyme and cloning, sequencing and analysis of the reductase genes

J Gen Microbiol. 1993 Aug;139(8):1817-28. doi: 10.1099/00221287-139-8-1817.


A dissimilatory sulphite reductase was isolated from the extremely thermophilic dissimilatory sulphate-reducing archaeon Archaeoglobus fulgidus. In common with other dissimilatory sulphite reductases thus far characterized, the enzyme has an alpha 2 beta 2-structure and contains sirohaem, non-haem iron atoms and acid labile sulphide. The oxidized enzyme exhibited absorption maxima at 281, 394, 545 and 593 nm with a weak band around 715 nm. We have cloned and sequenced the genes for the alpha and beta subunits of this enzyme, which we designate dsrA and dsrB, respectively. They are contiguous in the order dsrA dsrB and probably comprise an operon, since dsrA is preceded by sequences characteristic of promoters in methanogenic archaea, and dsrB is followed by a sequence resembling termination signals in extremely thermophilic sulphur-dependent archaea. dsrA and dsrB encode 47.4 kDa and 41.7 kDa peptides, which have 25.6% amino acid sequence identity, indicating that they may have arisen by duplication of an ancestral gene. Each deduced peptide contains cysteine clusters resembling those postulated to bind sirohaem-[Fe4S4] complexes in sulphite reductases and nitrite reductases from other species. The dsrB encoded peptide lacks a single cysteine residue in one of the two clusters, suggesting that only the alpha subunit binds a sirohaem-[Fe4S4] complex, and chemical analyses showed the presence of only two sirohaems per alpha 2 beta 2 enzyme molecule. Both deduced peptides also contain an arrangement of cysteine residues characteristic of [Fe4S4] ferredoxins, and chemical analyses were consistent with the presence of six [Fe4S4] clusters per alpha 2 beta 2 enzyme molecule, two of which would be expected to be associated with sirohaem while the other four could bind to the ferredoxin-like sites.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Archaea / enzymology
  • Archaea / genetics*
  • Base Sequence
  • Cloning, Molecular
  • DNA, Bacterial
  • Genes, Bacterial
  • Gram-Negative Anaerobic Bacteria / enzymology
  • Gram-Negative Anaerobic Bacteria / genetics*
  • Molecular Sequence Data
  • Oxidoreductases Acting on Sulfur Group Donors / genetics*
  • Oxidoreductases Acting on Sulfur Group Donors / isolation & purification
  • Oxidoreductases Acting on Sulfur Group Donors / metabolism
  • RNA, Bacterial
  • Sequence Homology, Amino Acid


  • DNA, Bacterial
  • RNA, Bacterial
  • Oxidoreductases Acting on Sulfur Group Donors

Associated data

  • GENBANK/M95624