The role of the O-antigen lipopolysaccharide on the colonization in vivo of the germfree chicken gut by Klebsiella pneumoniae

Microb Pathog. 1993 Jun;14(6):433-40. doi: 10.1006/mpat.1993.1042.


We isolated lipopolysaccharide and capsular polysaccharide (K antigen)-defective mutants from two Klebsiella pneumoniae parental strains, and compared their ability to colonize in vivo the germfree chicken gut. The high-molecular weight lipopolysaccharide (LPS) (O antigen) was found necessary for the colonization while the capsular polysaccharide (K2 or K29) was not of importance.

MeSH terms

  • Animals
  • Bacterial Capsules / analysis
  • Bacterial Capsules / physiology*
  • Chickens / microbiology*
  • Digestive System / microbiology
  • Feces / microbiology
  • Germ-Free Life
  • Klebsiella pneumoniae / chemistry*
  • Klebsiella pneumoniae / pathogenicity
  • Lipopolysaccharides* / analysis
  • Mutation
  • O Antigens
  • Polysaccharides, Bacterial / analysis
  • Polysaccharides, Bacterial / physiology*


  • Lipopolysaccharides
  • O Antigens
  • Polysaccharides, Bacterial