Chlorpromazine inhibits both the constitutive nitric oxide synthase and the induction of nitric oxide synthase after LPS challenge

Biochem Biophys Res Commun. 1993 Oct 15;196(1):280-6. doi: 10.1006/bbrc.1993.2246.


The effects of chlorpromazine on either the activity of mouse brain nitric oxide synthase or the induction of lung nitric oxide synthase in mice and rats were studied. Chlorpromazine inhibited the nitric oxide synthase activity in mouse brain cytosol. This effect could be reversed by adding an excess of calmodulin. In addition, chlorpromazine was able to inhibit the induction of lung nitric oxide synthase, in both species, after LPS administration. Furthermore, chlorpromazine also inhibited arginase activity in mouse lung cytosol.

MeSH terms

  • Amino Acid Oxidoreductases / biosynthesis*
  • Animals
  • Arginase / biosynthesis
  • Brain / drug effects*
  • Chlorpromazine / pharmacology*
  • Citrulline / biosynthesis
  • Cytosol / metabolism
  • Enzyme Induction / drug effects
  • Gene Expression Regulation, Enzymologic / drug effects*
  • Lipopolysaccharides / pharmacology
  • Lung / metabolism
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Nitric Oxide / biosynthesis
  • Nitric Oxide Synthase
  • Rats
  • Rats, Sprague-Dawley
  • Shock, Septic / etiology


  • Lipopolysaccharides
  • Citrulline
  • Nitric Oxide
  • Nitric Oxide Synthase
  • Amino Acid Oxidoreductases
  • Arginase
  • Chlorpromazine