Granulocyte colony-stimulating factor down-regulates the surface expression of the human leucocyte adhesion molecule-1 on human neutrophils in vitro and in vivo

Br J Haematol. 1993 Aug;84(4):574-80. doi: 10.1111/j.1365-2141.1993.tb03130.x.

Abstract

The leucocyte adhesion molecule-1 (LAM-1) is the human homologue of the murine peripheral lymph node homing receptor, MEL-14, and might play a crucial role in neutrophil localization at inflammatory sites. We have reported previously that recombinant human granulocyte colony-stimulating factor (rhG-CSF) stimulates or enhances several neutrophil functions in vivo, as well as in vitro. To further explore the possible role of G-CSF in inflammation we studied the effect of rhG-CSF on the surface expression of LAM-1 on human neutrophils, both in vitro and in vivo. The expression of LAM-1 by human neutrophils was investigated by indirect immunofluorescence using flow cytometry and monoclonal antibodies anti-Leu-8 and TQ1. A whole blood analysis was performed to minimize in vitro manipulation. Most circulating human neutrophils expressed LAM-1 on the cell surface. Brief exposure of neutrophils to rhG-CSF in vitro decreased the surface expression of LAM-1. rhG-CSF down-regulated neutrophil LAM-1 expression in a time- and dose-dependent manner. Neutrophils from healthy volunteers and from patients who were receiving rhG-CSF exhibited a decreased expression of LAM-1 after rhG-CSF administration, and the expression thereafter returned or overshot the pretreatment level after stopping rhG-CSF administration. These findings indicate that rhG-CSF down-regulates the surface expression of LAM-1 on human neutrophils in vivo, as well as in vitro, and G-CSF might participate in neutrophil-endothelial cell interaction in inflamed tissue.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, CD / drug effects
  • CD11 Antigens
  • Cell Adhesion / physiology
  • Cell Adhesion Molecules / drug effects*
  • Cell Adhesion Molecules / metabolism
  • Cells, Cultured
  • Dose-Response Relationship, Drug
  • Granulocyte Colony-Stimulating Factor / pharmacology*
  • Humans
  • In Vitro Techniques
  • L-Selectin
  • Membrane Glycoproteins / drug effects
  • Neutrophils / metabolism*
  • Recombinant Proteins / pharmacology
  • Time Factors

Substances

  • Antigens, CD
  • CD11 Antigens
  • Cell Adhesion Molecules
  • Membrane Glycoproteins
  • Recombinant Proteins
  • L-Selectin
  • Granulocyte Colony-Stimulating Factor