Abstract
We have identified a myelin basic protein (MBP) isoform in mouse embryos that includes an exon upstream of the usual transcription initiation site. This isoform, embryonic-neonatal MBP (E-MBP), is expressed at the protein level in the embryonic nervous system at a time when other MBP isoforms are not detected. In addition to the central and peripheral nervous systems of the embryo and neonate, the thymus, spleen, and testes also express E-MBP at the protein level. The expression of E-MBP in cell types distinct from the nervous system strongly suggests that this MBP isoform has a role apart from the formation of myelin.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Animals, Newborn
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Base Sequence
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Blotting, Western
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Brain / embryology
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Brain / metabolism*
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DNA Primers
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Embryo, Mammalian / metabolism*
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Exons
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Female
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Gene Expression
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Gestational Age
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Male
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Mice
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Mice, Inbred BALB C
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Molecular Sequence Data
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Molecular Weight
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Myelin Basic Protein / analysis
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Myelin Basic Protein / biosynthesis*
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Organ Specificity
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Polymerase Chain Reaction
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Pregnancy
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RNA, Messenger / analysis
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RNA, Messenger / biosynthesis*
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Sciatic Nerve / embryology
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Sciatic Nerve / metabolism*
Substances
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DNA Primers
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Myelin Basic Protein
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RNA, Messenger