Identification of an embryonic isoform of myelin basic protein that is expressed widely in the mouse embryo

Proc Natl Acad Sci U S A. 1993 Nov 1;90(21):10125-9. doi: 10.1073/pnas.90.21.10125.

Abstract

We have identified a myelin basic protein (MBP) isoform in mouse embryos that includes an exon upstream of the usual transcription initiation site. This isoform, embryonic-neonatal MBP (E-MBP), is expressed at the protein level in the embryonic nervous system at a time when other MBP isoforms are not detected. In addition to the central and peripheral nervous systems of the embryo and neonate, the thymus, spleen, and testes also express E-MBP at the protein level. The expression of E-MBP in cell types distinct from the nervous system strongly suggests that this MBP isoform has a role apart from the formation of myelin.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Animals, Newborn
  • Base Sequence
  • Blotting, Western
  • Brain / embryology
  • Brain / metabolism*
  • DNA Primers
  • Embryo, Mammalian / metabolism*
  • Exons
  • Female
  • Gene Expression
  • Gestational Age
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • Molecular Weight
  • Myelin Basic Protein / analysis
  • Myelin Basic Protein / biosynthesis*
  • Organ Specificity
  • Polymerase Chain Reaction
  • Pregnancy
  • RNA, Messenger / analysis
  • RNA, Messenger / biosynthesis*
  • Sciatic Nerve / embryology
  • Sciatic Nerve / metabolism*

Substances

  • DNA Primers
  • Myelin Basic Protein
  • RNA, Messenger