Hyaluronic-acid-deficient extracellular matrix induced by addition of 4-methylumbelliferone to the medium of cultured human skin fibroblasts

Biochem Biophys Res Commun. 1995 Mar 17;208(2):470-5. doi: 10.1006/bbrc.1995.1362.


The effects of xylosyl-beta-D-(4-methylumbelliferone) and its aglycone, 4-methylumbelliferone, on hyaluronic acid synthesis were investigated in cultured human skin fibroblasts. Xylosyl-beta-D-(4-methylumbelliferone) added to the medium of cultured cell reduced the synthesis of hyaluronic acid. Furthermore, 4-methylumbelliferone reduced the production of hyaluronic acid markedly. In addition, 4-methylumbelliferone had hardly any effect on proteoglycan synthesis, whereas xylosyl-beta-D-(4-methylumbelliferone) produced a large amount of glycosaminoglycan chains. The present results indicate that cells cultured with 4-methylumbelliferone produce a hyaluronic-acid-deficient extracellular matrix, which will be useful for functional studies of hyaluronic acid.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cells, Cultured
  • Extracellular Matrix / metabolism*
  • Fibroblasts / metabolism*
  • Glycosaminoglycans / biosynthesis
  • Humans
  • Hyaluronic Acid / metabolism*
  • Hymecromone / pharmacology*
  • In Vitro Techniques
  • Skin / cytology
  • Skin / metabolism


  • Glycosaminoglycans
  • Hymecromone
  • Hyaluronic Acid