Mice deficient for the IL-3/GM-CSF/IL-5 beta c receptor exhibit lung pathology and impaired immune response, while beta IL3 receptor-deficient mice are normal

Immunity. 1995 Mar;2(3):211-22. doi: 10.1016/1074-7613(95)90046-2.


The receptors for IL-3, GM-CSF, and IL-5 share a common beta subunit (beta c), and mice have an additional IL-3 beta subunit (beta IL3). We have independently generated mice carrying null mutations of each molecule. beta c mutant bone marrow showed no response to GM-CSF or IL-5, whereas IL-3 stimulation of beta c or beta IL3 mutant bone marrow was normal. beta c mutant mice showed lung pathology consisting of lymphocytic infiltration and areas resembling alveolar proteinosis, and also exhibited low basal numbers of eosinophils. Infection of beta c mutant mice by Nippostrongylus brasiliensis resulted in the absence of blood and lung eosinophilia. Animals repopulated with beta c mutant bone marrow cells showed slower leukocyte recovery and reduced eosinophil numbers. These data define the role of beta c in vivo, and show a phenotype that is likely to be the cumulative effect of loss of GM-CSF and IL-5 stimulation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Bone Marrow Transplantation / immunology
  • Eosinophils / immunology
  • Eosinophils / pathology*
  • Flow Cytometry
  • Lung Diseases / immunology
  • Lung Diseases / pathology*
  • Mice
  • Mice, Knockout
  • Molecular Sequence Data
  • Nippostrongylus / immunology
  • Receptors, Cytokine / deficiency
  • Receptors, Cytokine / genetics
  • Receptors, Cytokine / physiology*
  • Receptors, Granulocyte-Macrophage Colony-Stimulating Factor / physiology
  • Receptors, Interleukin / physiology
  • Receptors, Interleukin-3 / physiology
  • Receptors, Interleukin-5
  • Strongylida Infections / immunology


  • Receptors, Cytokine
  • Receptors, Granulocyte-Macrophage Colony-Stimulating Factor
  • Receptors, Interleukin
  • Receptors, Interleukin-3
  • Receptors, Interleukin-5