Cytokine-induced differentiation of precursor mouse CD8+ T cells into cytotoxic CD8+ T cells secreting Th1 or Th2 cytokines

Immunity. 1995 Mar;2(3):271-9. doi: 10.1016/1074-7613(95)90051-9.


Alloantigen-stimulated CD8+ mouse spleen cells, either spontaneously or in the presence of IL-12 or IFN gamma plus anti-IL-4, differentiate into CD8+ T cells secreting a Th1-like cytokine pattern (IL-2 and IFN gamma). IL-4 induced differentiation into CD8+ T cells secreting Th2 cytokines (IL-4, IL-5, IL-6, and IL-10), whereas anti-IFN gamma suppressed the development of CD8+ cells secreting IFN gamma. Clones of IL-4- or IFN gamma-producing CD8+ T cells were relatively stable, as IL-4 or IFN gamma did not cause interconversion of committed CD8+ T cells. Both CD8+ subsets were cytotoxic, failed to provide cognate help for B cell antibody production, and remained CD4-, CD8 alpha+ CD8 beta+. We propose the names TC1 and TC2 for cytotoxic CD8+ T cells secreting Th1-like and Th2-like cytokines, respectively.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • B-Lymphocytes / immunology
  • Cell Differentiation / immunology*
  • Cell Line
  • Cytotoxicity Tests, Immunologic
  • Female
  • Flow Cytometry
  • Immunoglobulin Isotypes / immunology
  • Interleukin-4 / physiology*
  • Interleukins / biosynthesis
  • Interleukins / metabolism*
  • Lymphocyte Cooperation / immunology
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Mice, Inbred CBA
  • T-Lymphocytes, Cytotoxic / immunology*
  • Th1 Cells / immunology
  • Th2 Cells / immunology


  • Immunoglobulin Isotypes
  • Interleukins
  • Interleukin-4