An Absolute Requirement for Both the Type II and Type I Receptors, Punt and Thick Veins, for DPP Signaling in Vivo

Cell. 1995 Mar 24;80(6):889-97. doi: 10.1016/0092-8674(95)90292-9.

Abstract

TGF beta elicits diverse cellular responses by signaling through receptor complexes formed by two distantly related transmembrane serine/threonine kinases called type II and type I receptors. Previous studies have indicated that the product of the Drosophila thick veins (tkv) gene is a type I receptor for decapentaplegic (dpp). Here, we show that the Drosophila gene punt encodes a homolog of a vertebrate type II receptor, and we demonstrate that punt, like tkv, is essential in vivo for dpp-dependent patterning processes. Because no dpp-related signalling is apparent in the absence of either the punt or tkv receptor, we infer that both receptors act in concert to transduce the dpp signal and that their functions cannot be replaced by the other extant type II and I receptors.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Activin Receptors
  • Alleles
  • Animals
  • Animals, Genetically Modified
  • Drosophila / embryology
  • Drosophila / genetics*
  • Drosophila Proteins*
  • Embryo, Nonmammalian / physiology
  • Genes, Insect*
  • Homozygote
  • Insect Hormones / genetics
  • Insect Hormones / metabolism*
  • Molecular Sequence Data
  • Protein-Serine-Threonine Kinases / biosynthesis
  • Protein-Serine-Threonine Kinases / metabolism*
  • Receptors, Cell Surface / biosynthesis
  • Receptors, Cell Surface / metabolism*
  • Receptors, Growth Factor / biosynthesis
  • Receptors, Growth Factor / metabolism*
  • Signal Transduction
  • Transcription, Genetic
  • Transforming Growth Factor beta / metabolism
  • Vertebrates

Substances

  • Drosophila Proteins
  • Insect Hormones
  • Receptors, Cell Surface
  • Receptors, Growth Factor
  • Transforming Growth Factor beta
  • dpp protein, Drosophila
  • tkv protein, Drosophila
  • Protein-Serine-Threonine Kinases
  • Activin Receptors

Associated data

  • GENBANK/L38495