The goal of athletic training is to provide the body with a stimulus to adapt, increasing the capacity of the various systems to perform increased work loads. However, the magnitude of the stress must be large enough to induce the synthesis of new enzymes, tissues, and yet not so large that the biochemical and physiological processes of recovery are depressed. As each individual undergoes this process at a different rate, objective evaluation of the level of fatigue and adaptation is of enormous value in directing the training program of an athlete. The role of iron in the body is such that this element can be used as a marker of both adaptation to training and as an indicator of an acute inflammatory response to exercise. The various clinical measurements of iron in transport, storage, and in hematological parameters are discussed in this paper, relative to athletic populations. In addition expected changes in the level of the acute inflammatory protein, alpha 1-antitrypsin are also discussed relative to acute and chronic training protocols. Data is presented indicating that measurement of serum iron, transferrin, serum ferritin, and alpha 1-antitrypsin can be used to differentiate between an inflammatory response to tissue damage and infection. These parameters can also provide information as to the state of recovery, or lack thereof, experienced by an athlete to both acute and chronic training programs. The use of biochemical markers can help to avoid an overstress situation.