Accumulation and cellular localization of fibrinogen/fibrin during short-term and long-term rat liver injury

Gastroenterology. 1995 Apr;108(4):1124-35. doi: 10.1016/0016-5085(95)90211-2.


Background/aims: During liver fibrosis, there is a putative pacemaker role of fibronectin. Fibrinogen is closely linked to fibronectin during clotting processes. The aim of this study was to show fibrinogen gene expression during liver damage.

Methods: Fibrinogen/fibrin deposition in damaged livers was studied by immunohistology. Fibrinogen gene expression was analyzed in vivo in a model of CCl4-induced rat liver damage and in vitro in isolated liver cells by means of Northern blot analysis and in situ hybridization.

Results: Immunohistology showed striking amounts of fibrinogen and fibrin deposits in pericentral necrotic areas (short-term damage) and within fibrotic septa (long-term damage). Total RNA extracted from short-term-damaged livers contained an increased fibrinogen messenger RNA level. By in situ hybridization, fibrinogen transcripts were localized in cells of the nonnecrotic areas (short-term damage) and outside fibrotic septa (long-term damage). In vitro studies showed fibrinogen de novo synthesis restricted to hepatocytes.

Conclusions: The results show fibrinogen/fibrin deposition during short-term liver injury and liver fibrogenesis, which may suggest the involvement of a "clotting-like process" in short-term liver damage and liver fibrosis. The data might indicate that fibrin/fibronectin constitute a "provisional matrix," which affects the attraction and proliferation of inflammatory and matrix-producing cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Northern
  • Carbon Tetrachloride
  • Chemical and Drug Induced Liver Injury
  • Female
  • Fibrin / metabolism*
  • Fibrinogen / biosynthesis
  • Fibrinogen / genetics
  • Fibrinogen / metabolism*
  • Gene Expression
  • Immunohistochemistry
  • In Situ Hybridization
  • Liver / metabolism*
  • Liver / pathology
  • Liver Cirrhosis, Experimental / chemically induced
  • Liver Cirrhosis, Experimental / metabolism
  • Liver Diseases / metabolism*
  • Liver Diseases / pathology
  • Necrosis
  • RNA, Messenger / metabolism
  • Rats
  • Rats, Wistar


  • RNA, Messenger
  • Fibrin
  • Fibrinogen
  • Carbon Tetrachloride