Requirement for Lim1 in Head-Organizer Function

Nature. 1995 Mar 30;374(6521):425-30. doi: 10.1038/374425a0.

Abstract

Lim1 is a homeobox gene expressed in the organizer region of mouse embryos. To investigate the role of Lim1 during embryogenesis, a targeted deletion of the Lim1 gene was generated in embryonic stem cells. Embryos homozygous for the null allele lacked anterior head structures but the remaining body axis developed normally. A partial secondary axis developed anteriorly in some mutant embryos. Lim1 is thus an essential regulator of the vertebrate head organizer.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • Biomarkers
  • Brain / cytology
  • Brain / embryology
  • DNA Primers
  • Embryonic Induction / physiology*
  • Embryonic and Fetal Development / genetics
  • Embryonic and Fetal Development / physiology*
  • Gene Deletion
  • Gene Expression
  • Gene Targeting
  • Genes, Homeobox
  • Head / embryology*
  • Homeodomain Proteins / genetics
  • Homeodomain Proteins / physiology*
  • LIM-Homeodomain Proteins
  • Mesoderm
  • Mice
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • Phenotype
  • Stem Cells
  • Transcription Factors

Substances

  • Biomarkers
  • DNA Primers
  • Homeodomain Proteins
  • LIM-Homeodomain Proteins
  • Lhx1 protein, mouse
  • Transcription Factors