Polyadenylation of c-mos mRNA as a control point in Xenopus meiotic maturation

Nature. 1995 Apr 6;374(6522):511-6. doi: 10.1038/374511a0.

Abstract

c-mos protein, encoded by a proto-oncogene, is essential for the meiotic maturation of frog oocytes. Polyadenylation of c-mos messenger RNA is shown here to be a pivotal regulatory step in meiotic maturation. Maturation is prevented by selective amputation of polyadenylation signals from c-mos mRNA. Injection of a prosthetic RNA, which restores c-mos polyadenylation signals by base pairing to the amputated mRNA, rescues maturation and can stimulate translation in trans. Prosthetic RNAs may provide a general strategy by which to alter patterns of mRNA expression in vivo.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • DNA
  • Meiosis / genetics*
  • Molecular Sequence Data
  • Oligonucleotides, Antisense / pharmacology
  • Oocytes / cytology
  • Poly A / metabolism*
  • Progesterone / physiology
  • Protein Biosynthesis / physiology
  • Proto-Oncogene Proteins c-mos / genetics*
  • RNA, Messenger / chemical synthesis
  • RNA, Messenger / metabolism*
  • RNA, Messenger / pharmacology
  • Xenopus

Substances

  • Oligonucleotides, Antisense
  • RNA, Messenger
  • Poly A
  • Progesterone
  • DNA
  • Proto-Oncogene Proteins c-mos