Antioxidant function of recombinant human natural killer enhancing factor

Biochem Biophys Res Commun. 1995 Mar 28;208(3):964-9. doi: 10.1006/bbrc.1995.1428.

Abstract

Natural killer enhancing factor (NKEF) is a member of a new class of highly conserved antioxidant proteins. Members of this family have been described as thiol-specific antioxidants. In this study, we show that recombinant proteins encoded by the two human NKEF genes (nkef-A and B) possess antioxidant function in the protection of protein and DNA from oxidative damage. The production of separate proteins from each of the genes encoding NKEF is an important step in the elucidation of its function.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antioxidants / pharmacology*
  • Blood Proteins / biosynthesis
  • Blood Proteins / isolation & purification
  • Blood Proteins / pharmacology*
  • Blotting, Western
  • DNA Damage
  • DNA, Complementary / isolation & purification
  • Electrophoresis, Polyacrylamide Gel
  • Gene Expression
  • Glutamate-Ammonia Ligase / antagonists & inhibitors
  • Glutamate-Ammonia Ligase / metabolism*
  • Heat-Shock Proteins
  • Humans
  • Killer Cells, Natural / physiology
  • Kinetics
  • Peroxidases
  • Peroxiredoxins
  • Plasmids / drug effects*
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / isolation & purification
  • Recombinant Proteins / pharmacology*

Substances

  • Antioxidants
  • Blood Proteins
  • DNA, Complementary
  • Heat-Shock Proteins
  • Recombinant Proteins
  • Peroxidases
  • PRDX2 protein, human
  • Peroxiredoxins
  • Glutamate-Ammonia Ligase