Abstract
We previously reported that human chromosome 1q21 or 1q23-q24 carries a transformation suppressor gene(s) for Kirsten sarcoma virus-transformed NIH3T3 cells. In this study, we have isolated a novel human cDNA on 1q21 (designated as YL-1) as a candidate, then cloned its mouse homolog. Human and mouse YL-1 cDNA shared 96% in amino acid homology and presumably encoded a transcription factor-like polypeptide. An analysis using a specific antibody revealed the nuclear localization of YL-1 protein. The bacterially expressed YL-1 could bind to DNA. Thus, YL-1 protein possibly functions as a transcriptional regulator.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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3T3 Cells
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Amino Acid Sequence
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Animals
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Base Sequence
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Chromosome Mapping*
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Chromosomes, Human, Pair 1*
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Cloning, Molecular
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DNA-Binding Proteins / biosynthesis
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DNA-Binding Proteins / genetics*
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Genes, Suppressor
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Hominidae / genetics*
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Humans
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Immunoblotting
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Mice / genetics*
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Molecular Sequence Data
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Nuclear Proteins / biosynthesis
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Repressor Proteins*
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Sequence Homology, Amino Acid
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Transcription Factors / biosynthesis
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Transcription Factors / genetics*
Substances
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DNA-Binding Proteins
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Nuclear Proteins
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Repressor Proteins
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Transcription Factors
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VPS72 protein, human
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Vps72 protein, mouse
Associated data
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GENBANK/D43642
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GENBANK/D43643