Molecular cloning of a novel human cDNA on chromosome 1q21 and its mouse homolog encoding a nuclear protein with DNA-binding ability

Biochem Biophys Res Commun. 1995 Mar 28;208(3):999-1007. doi: 10.1006/bbrc.1995.1433.


We previously reported that human chromosome 1q21 or 1q23-q24 carries a transformation suppressor gene(s) for Kirsten sarcoma virus-transformed NIH3T3 cells. In this study, we have isolated a novel human cDNA on 1q21 (designated as YL-1) as a candidate, then cloned its mouse homolog. Human and mouse YL-1 cDNA shared 96% in amino acid homology and presumably encoded a transcription factor-like polypeptide. An analysis using a specific antibody revealed the nuclear localization of YL-1 protein. The bacterially expressed YL-1 could bind to DNA. Thus, YL-1 protein possibly functions as a transcriptional regulator.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3 Cells
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Chromosome Mapping*
  • Chromosomes, Human, Pair 1*
  • Cloning, Molecular
  • DNA-Binding Proteins / biosynthesis
  • DNA-Binding Proteins / genetics*
  • Genes, Suppressor
  • Hominidae / genetics*
  • Humans
  • Immunoblotting
  • Mice / genetics*
  • Molecular Sequence Data
  • Nuclear Proteins / biosynthesis
  • Repressor Proteins*
  • Sequence Homology, Amino Acid
  • Transcription Factors / biosynthesis
  • Transcription Factors / genetics*


  • DNA-Binding Proteins
  • Nuclear Proteins
  • Repressor Proteins
  • Transcription Factors
  • VPS72 protein, human
  • Vps72 protein, mouse

Associated data

  • GENBANK/D43642
  • GENBANK/D43643