Plant constituents interfering with human sex hormone-binding globulin. Evaluation of a test method and its application to Urtica dioica root extracts

Z Naturforsch C J Biosci. Jan-Feb 1995;50(1-2):98-104.

Abstract

A test system is described, which allows the search for compounds interfering with human sex hormone-binding globulin (SHBG) even in complex plant extracts. The method has been evaluated and applied to Urtica dioica root extracts. The lignan secoisolariciresinol (5) as well as a mixture of isomeric (11 E)-9,10,13-trihydroxy-11-octadecenoic and (10 E)-9,12,13-trihydroxy-10-octadecenoic acids (3 and 4, resp.) were demonstrated to reduce binding activity of human SHBG. Methylation of the mixture of 3 and 4 increased its activity about 10-fold.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Dihydrotestosterone / metabolism
  • Female
  • Humans
  • Hydroxy Acids / chemistry
  • Hydroxy Acids / isolation & purification
  • Hydroxy Acids / pharmacology*
  • Kinetics
  • Methylation
  • Molecular Structure
  • Oleic Acids / chemistry
  • Oleic Acids / isolation & purification
  • Oleic Acids / pharmacology*
  • Plant Extracts / pharmacology*
  • Plant Roots
  • Plants, Medicinal*
  • Pregnancy
  • Pregnancy Trimester, Third
  • Protein Binding
  • Serum Albumin / isolation & purification
  • Serum Albumin / metabolism
  • Sex Hormone-Binding Globulin / antagonists & inhibitors*
  • Sex Hormone-Binding Globulin / isolation & purification

Substances

  • Hydroxy Acids
  • Oleic Acids
  • Plant Extracts
  • Serum Albumin
  • Sex Hormone-Binding Globulin
  • Dihydrotestosterone
  • 9,10,13-trihydroxy-11-octadecenoic acid