Highly discriminating heptaplex short tandem repeat PCR system for forensic identification

Biotechniques. 1995 Jan;18(1):116-8, 120-1.


We describe a highly discriminating multiplex short tandem repeat PCR human identification system that gives a matching probability for Caucasians of European ancestry of 2.94 x 10(-8) or 5.66 x 10(-10) when used in combination with a previously described system. The system produces discrimination equal to or greater than four single locus probes (restriction fragment length polymorphism [RFLP] typing of variable nucleotide tandem repeat [VNTR] loci). The test is robust and reproducible and works with 1-10 ng of template DNA, using fluorescent detection of PCR products from either 4 or 6 short tandem repeat loci and the X-Y homologous gene amelogenin, giving simultaneous sex diagnosis.

MeSH terms

  • Alleles
  • Base Sequence
  • Chromosome Mapping
  • Forensic Medicine*
  • Humans
  • Molecular Sequence Data
  • Polymerase Chain Reaction / methods*
  • Repetitive Sequences, Nucleic Acid*
  • Sex Determination Analysis*