Fast separations of plasmid DNA using discontinuous gradients in the preparative ultracentrifuge

M Dorin; C A Bornecque

Fast separations of plasmid DNA using discontinuous gradients in the preparative ultracentrifuge

Biotechniques. 1995 Jan;18(1):90, 92.

Authors

M Dorin¹, C A Bornecque

Affiliation

¹ Research and Applications Department, Beckman Instruments, Palo Alto, CA 94304.

PMID: 7702860

Abstract

Plasmid DNA can be purified in 20 min using a three-step CsCl gradient in a vertical tube rotor. Ultracentrifugation with discontinuous gradients yields DNA with purity comparable to that obtained with longer isopycnic separations and with columns.

Publication types

Technical Report

MeSH terms

Centrifugation, Density Gradient
DNA / isolation & purification*
Plasmids*
Ultracentrifugation

Substances

DNA