BDNF and NT-4/5 enhance glutamatergic synaptic transmission in cultured hippocampal neurones

Neuroreport. 1994 Dec 30;6(1):21-5. doi: 10.1097/00001756-199412300-00007.


The effects of BDNF and NT-4/5 on AMPA receptor-mediated synaptic transmission were investigated with the patch clamp technique applied to embryonic and post-natal rat hippocampal neurones, cultured in serum-free medium. Spatially restricted application of neurotrophin-containing solution on to the recorded cells was performed and evoked as well as miniature excitatory postsynaptic currents (mepscs) were monitored. In approximately 25% of neurones tested a transient augmentation of evoked synaptic currents and a transient increase in the frequency of mepscs occurred with a delay of 0.5-5 min after the onset of BDNF or NT-4/5 application. The amplitudes of the AMPA receptor mediated mepscs were unaffected, suggesting a presynaptic action of BDNF and NT-4/5.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Newborn
  • Brain-Derived Neurotrophic Factor
  • Cells, Cultured
  • Electrophysiology
  • Fetus
  • Glutamic Acid / physiology*
  • Hippocampus / cytology
  • Hippocampus / physiology*
  • Nerve Growth Factors / pharmacology*
  • Nerve Tissue Proteins / pharmacology*
  • Neurons / physiology
  • Rats
  • Synaptic Transmission / drug effects*


  • Brain-Derived Neurotrophic Factor
  • Nerve Growth Factors
  • Nerve Tissue Proteins
  • neurotrophin 5
  • Glutamic Acid
  • neurotrophin 4