Determination of semicarbazide-sensitive amine oxidase activity in human plasma by high-performance liquid chromatography with fluorimetric detection

J Chromatogr B Biomed Appl. 1995 Jan 6;663(1):43-50. doi: 10.1016/0378-4347(94)00408-w.

Abstract

We report here a simple and sensitive method for the measurement of semicarbazide-sensitive amine oxidase (SSAO) activity in human plasma. Benzaldehyde, generated during a 1-h incubation of plasma with benzylamine, is derivatized with the specific aldehyde reagent dimedone after prior deproteinization. Quantitation of the derivatization product is done by automated injection onto an isocratic high-performance liquid chromatographic system with fluorimetric detection. The assay shows good linearity and reproducibility (intra-assay C.V. 7%). Detection limit is 25 mU/l (= pmol/ml/min). In 51 healthy controls (age 49 +/- 13 yr, 20 males) the measured SSAO activity was 352 +/- 102 mU/l (mean +/- S.D.). A large number of samples (70-80) can easily be processed in one day by one technician.

MeSH terms

  • Adult
  • Amine Oxidase (Copper-Containing)*
  • Benzaldehydes / analysis
  • Benzaldehydes / metabolism
  • Benzylamines / metabolism
  • Chromatography, High Pressure Liquid / methods*
  • Chromatography, High Pressure Liquid / standards
  • Chromatography, High Pressure Liquid / statistics & numerical data
  • Cyclohexanones
  • Female
  • Humans
  • Hydrogen-Ion Concentration
  • Male
  • Middle Aged
  • Oxidoreductases Acting on CH-NH Group Donors / blood*
  • Reference Values
  • Reproducibility of Results
  • Semicarbazides / pharmacology*

Substances

  • Benzaldehydes
  • Benzylamines
  • Cyclohexanones
  • Semicarbazides
  • carbamylhydrazine
  • benzylamine
  • dimedone
  • Amine Oxidase (Copper-Containing)
  • Oxidoreductases Acting on CH-NH Group Donors
  • benzaldehyde