Complete nucleotide sequence of a skin element excised by DNA rearrangement during sporulation in Bacillus subtilis

Microbiology. 1995 Feb;141 ( Pt 2):323-7. doi: 10.1099/13500872-141-2-323.

Abstract

As part of the Bacillus subtilis genome sequencing project, we have determined the complete nucleotide sequence of a skin element which is located between spoIVCB and spoIIIC. The entire sequence of this element is 48,032 bp in length, and contains 57 ORFs with putative ribosome-binding sites. Two of them correspond to previously sequenced and characterized genes, cwIA and spoIVCA. Furthermore, seven ORF products identified in this element show interesting similarities with known proteins present in data banks, including the phi 105 immunity repressor, the phi 105 Cro-like protein and the SPP1 terminase. These results indicate the possibility that the skin element is a cryptic remnant of an ancestral temperate phage.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Bacillus Phages / genetics
  • Bacillus subtilis / genetics*
  • Bacillus subtilis / growth & development
  • Base Sequence
  • Cloning, Molecular
  • DNA Transposable Elements / genetics*
  • DNA, Bacterial / genetics*
  • Gene Rearrangement
  • Molecular Sequence Data
  • Open Reading Frames / genetics
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Spores, Bacterial / genetics
  • Spores, Bacterial / growth & development
  • Transcription Factors / genetics*

Substances

  • DNA Transposable Elements
  • DNA, Bacterial
  • Transcription Factors
  • sigma K

Associated data

  • GENBANK/D32216