Primary structure of the neuronal clathrin-associated protein auxilin and its expression in bacteria

Eur J Biochem. 1995 Mar 1;228(2):297-304.

Abstract

The protein auxilin is a coat component of brain clathrin-coated vesicles. It interacts directly with the heavy chain of clathrin and supports its assembly into regular cages [Ahle, S. & Ungewickell, E. (1990) J. Cell Biol. 111, 19-29]. The combined open reading frames of three cow brain cDNA clones with a total of 4531 nucleotides predict a molecular mass of 99,504 Da for auxilin. The coding region is followed by a very long untranslated region of at least 1670 nucleotides. By Northern analysis, auxilin transcripts are found only in brain tissue. Auxilin is not related to any of the previously sequenced clathrin-binding proteins, but the region of positions 50-350 is 29% identical (similarity 56%) to the corresponding region of the actin-binding protein tensin from chicken fibroblasts. Recombinant auxilin expressed in and purified from bacteria by affinity chromatography is functional with respect to clathrin binding.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Vesicular Transport
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cattle
  • Chickens
  • Clathrin / chemistry*
  • Cloning, Molecular
  • Escherichia coli / genetics
  • Microfilament Proteins / chemistry
  • Molecular Sequence Data
  • Nerve Tissue Proteins / biosynthesis
  • Nerve Tissue Proteins / chemistry*
  • Nerve Tissue Proteins / genetics
  • Phosphoproteins / biosynthesis
  • Phosphoproteins / chemistry*
  • Phosphoproteins / genetics
  • RNA, Messenger / analysis
  • Recombinant Proteins / biosynthesis
  • Tensins

Substances

  • Adaptor Proteins, Vesicular Transport
  • Clathrin
  • Microfilament Proteins
  • Nerve Tissue Proteins
  • Phosphoproteins
  • RNA, Messenger
  • Recombinant Proteins
  • Tensins

Associated data

  • GENBANK/M78861
  • GENBANK/U09237