[Value of the adenosine triphosphate chemosensitivity assay for pre-therapeutic determination of the chemosensitivity of breast cancer cells in cell culture]

Geburtshilfe Frauenheilkd. 1995 Jan;55(1):7-16. doi: 10.1055/s-2007-1022766.
[Article in German]

Abstract

This presentation gives a summary of the chemosensitivity test results in breast cancer using the ATP-Cell-Viability Assay (ATP-CVA). Other assays and test systems for chemosensitivity testing are also evaluated. Our results with the ATP-CVA in breast cancer cell lines showed that this test system is a reproducible and reliable tool in the screening of new drugs. Our results with drug combinations showed that with the ATP-CVA and the "median effect principle" synergistic and antagonistic effects can be reliably calculated. The application of the ATP- CVA in fresh human breast cancer made it possible to test tumor chemosensitivity in individual patients. We found an evaluability rate of the ATP-CVA of 95% in 116 patients. The chemosensitivity profiles of the tested tumors revealed a big heterogeneity of chemosensitivity results for single and drug combinations. The 22 in vitro/in vivo correlations from patients with metastatic breast cancer treated with at least three cycles of chemotherapy showed a sensitivity of the ATP-CVA of 92% and a specificity of 89%.

Conclusions: These data are encouraging and further investigations on chemosensitivity testing of fresh tumours are currently ongoing in a prospective study at the University of Zurich.

Publication types

  • English Abstract

MeSH terms

  • Adenosine Triphosphate / metabolism*
  • Antineoplastic Combined Chemotherapy Protocols / therapeutic use*
  • Breast Neoplasms / drug therapy*
  • Breast Neoplasms / pathology
  • Cell Division / drug effects
  • Cell Line
  • Cell Survival / drug effects*
  • Dose-Response Relationship, Drug
  • Drug Resistance, Multiple
  • Drug Screening Assays, Antitumor*
  • Energy Metabolism / drug effects
  • Female
  • Humans
  • Reproducibility of Results
  • Tumor Cells, Cultured / drug effects*
  • Tumor Stem Cell Assay

Substances

  • Adenosine Triphosphate