Cyclosporin A inhibits 2-chloroadenosine-induced DNA cleavage in mouse thymocytes

Int J Immunopharmacol. 1994 Dec;16(12):995-1001. doi: 10.1016/0192-0561(94)90078-7.


Incubation of mouse thymocytes with the adenosine analogue 2-chloroadenosine resulted in enhanced internucleosomal DNA fragmentation which could be inhibited by the immunosuppressive drug cyclosporin A. In order to be effective, cyclosporin A had to be added to thymocyte preparations at the same time as 2-chloroadenosine. Since cyclosporin A is a selective inhibitor of calcineurin, our data suggest a possible role for calcineurin as a signaling intermediate in the apoptotic pathway activated in thymocytes through adenosine receptors. However, at the present time we cannot exclude the possibility that the inhibitory effect of cyclosporin A on 2-chloroadenosine-induced apoptosis may be mediated through a calcineurin-independent process.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 2-Chloroadenosine / antagonists & inhibitors*
  • Animals
  • Apoptosis / drug effects*
  • Calcineurin
  • Calmodulin-Binding Proteins / physiology
  • Cyclosporine / pharmacology*
  • DNA / metabolism*
  • DNA-Binding Proteins / physiology
  • Male
  • Mice
  • Mice, Inbred C57BL
  • NFATC Transcription Factors
  • Nuclear Proteins*
  • Phosphoprotein Phosphatases / physiology
  • T-Lymphocytes / drug effects*
  • T-Lymphocytes / physiology
  • Transcription Factors / physiology


  • Calmodulin-Binding Proteins
  • DNA-Binding Proteins
  • NFATC Transcription Factors
  • Nuclear Proteins
  • Transcription Factors
  • 2-Chloroadenosine
  • Cyclosporine
  • DNA
  • Calcineurin
  • Phosphoprotein Phosphatases