Mutant genes of gramicidin S synthetase 1 defective in phenylalanine racemization have the same sequence as the wild gene

J Biochem. 1994 Dec;116(6):1202-4. doi: 10.1093/oxfordjournals.jbchem.a124663.

Abstract

Mutant grs1 genes were cloned and sequenced from the Bacillus brevis Nagano BI-4, C-3, E-1, and E-2 strains, which produce defective gramicidin S synthetase 1 (GS1), lacking racemase activity. Surprisingly, these mutant genes had entirely the same sequence as that of the wild type gene. These mutant strains also produce defective gramicidin S synthetase 2 (GS2), lacking 4'-phosphopantetheine, a prosthetic group of this enzyme. The participation of this group in phenylalanine racemization is suggested.

MeSH terms

  • Amino Acid Isomerases / chemistry
  • Amino Acid Isomerases / genetics*
  • Amino Acid Isomerases / metabolism
  • Amino Acid Sequence
  • Bacillus / enzymology*
  • Bacillus / genetics*
  • Bacterial Proteins / chemistry
  • Bacterial Proteins / genetics*
  • Bacterial Proteins / metabolism
  • Cloning, Molecular
  • Molecular Sequence Data
  • Multienzyme Complexes / chemistry
  • Multienzyme Complexes / genetics*
  • Multienzyme Complexes / metabolism
  • Mutation* / genetics
  • Peptide Synthases / chemistry
  • Peptide Synthases / genetics*
  • Peptide Synthases / metabolism
  • Phenylalanine / chemistry*
  • Phenylalanine / metabolism
  • Racemases and Epimerases / genetics
  • Racemases and Epimerases / metabolism
  • Stereoisomerism
  • Structure-Activity Relationship

Substances

  • Bacterial Proteins
  • Multienzyme Complexes
  • Phenylalanine
  • Racemases and Epimerases
  • Amino Acid Isomerases
  • Peptide Synthases
  • gramicidin-S-synthetase 2