Abstract
Mutant grs1 genes were cloned and sequenced from the Bacillus brevis Nagano BI-4, C-3, E-1, and E-2 strains, which produce defective gramicidin S synthetase 1 (GS1), lacking racemase activity. Surprisingly, these mutant genes had entirely the same sequence as that of the wild type gene. These mutant strains also produce defective gramicidin S synthetase 2 (GS2), lacking 4'-phosphopantetheine, a prosthetic group of this enzyme. The participation of this group in phenylalanine racemization is suggested.
MeSH terms
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Amino Acid Isomerases / chemistry
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Amino Acid Isomerases / genetics*
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Amino Acid Isomerases / metabolism
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Amino Acid Sequence
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Bacillus / enzymology*
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Bacillus / genetics*
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Bacterial Proteins / chemistry
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Bacterial Proteins / genetics*
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Bacterial Proteins / metabolism
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Cloning, Molecular
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Molecular Sequence Data
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Multienzyme Complexes / chemistry
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Multienzyme Complexes / genetics*
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Multienzyme Complexes / metabolism
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Mutation* / genetics
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Peptide Synthases / chemistry
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Peptide Synthases / genetics*
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Peptide Synthases / metabolism
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Phenylalanine / chemistry*
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Phenylalanine / metabolism
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Racemases and Epimerases / genetics
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Racemases and Epimerases / metabolism
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Stereoisomerism
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Structure-Activity Relationship
Substances
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Bacterial Proteins
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Multienzyme Complexes
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Phenylalanine
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Racemases and Epimerases
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Amino Acid Isomerases
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Peptide Synthases
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gramicidin-S-synthetase 2