Intranasal Immunization With Recombinant Group A Streptococcal M Protein Fragment Fused to the B Subunit of Escherichia Coli Labile Toxin Protects Mice Against Systemic Challenge Infections

J Infect Dis. 1995 Apr;171(4):1038-41. doi: 10.1093/infdis/171.4.1038.

Abstract

A fusion gene named LT-B-M5 was constructed encoding the entire B subunit of Escherichia coli labile toxin (LT-B), a 7 amino acid proline-rich linker, and 15 amino-terminal amino acids of type 5 streptococcal M protein. The purified LT-B-M5 was immunogenic in rabbits and evoked antibodies against a synthetic peptide copy of the amino-terminus of M5 (SM5[1-15]) and the native M5 protein and opsonic antibodies against type 5 streptococci. The hybrid protein retained the ganglioside-binding activity of LT-B and was tested in mice for its immunogenicity after local administration. Mice that were immunized intranasally with LT-B-M5 developed serum antibodies against SM5(1-15) and were significantly protected from death after intraperitoneal challenge infections with type 5 streptococci. The data show that protective systemic immune responses may be evoked after intranasal immunization with a fragment of M protein fused to LT-B.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Administration, Intranasal
  • Animals
  • Antibodies, Bacterial / biosynthesis
  • Antibodies, Bacterial / blood
  • Antigens, Bacterial / immunology*
  • Bacterial Outer Membrane Proteins*
  • Bacterial Proteins / genetics
  • Bacterial Proteins / immunology*
  • Bacterial Toxins / genetics
  • Bacterial Toxins / immunology*
  • Bacterial Toxins / metabolism
  • Bacterial Vaccines / administration & dosage
  • Bacterial Vaccines / immunology*
  • Base Sequence
  • Carrier Proteins*
  • Enterotoxins / genetics
  • Enterotoxins / immunology*
  • Enterotoxins / metabolism
  • Escherichia coli / genetics
  • Escherichia coli Proteins*
  • Female
  • Gangliosides / metabolism
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • Rabbits
  • Recombinant Fusion Proteins / administration & dosage
  • Recombinant Fusion Proteins / immunology*
  • Recombinant Fusion Proteins / isolation & purification
  • Recombinant Fusion Proteins / metabolism
  • Streptococcal Infections / prevention & control*
  • Streptococcus / immunology*
  • Vaccination
  • Vaccines, Synthetic / administration & dosage
  • Vaccines, Synthetic / immunology

Substances

  • Antibodies, Bacterial
  • Antigens, Bacterial
  • Bacterial Outer Membrane Proteins
  • Bacterial Proteins
  • Bacterial Toxins
  • Bacterial Vaccines
  • Carrier Proteins
  • Enterotoxins
  • Escherichia coli Proteins
  • Gangliosides
  • Recombinant Fusion Proteins
  • Vaccines, Synthetic
  • heat-labile enterotoxin, E coli
  • streptococcal M protein