Protection of dogs from Lyme disease with a vaccine containing outer surface protein (Osp) A, OspB, and the saponin adjuvant QS21

J Infect Dis. 1995 Apr;171(4):1049-52. doi: 10.1093/infdis/171.4.1049.

Abstract

A vaccine consisting of purified Escherichia coli-expressed recombinant full-length outer surface proteins A (OspA) and B (OspB) and the saponin adjuvant QS21 was evaluated for protection against Borrelia burgdorferi infection. Eleven beagles were vaccinated twice and then challenged with 10 field-collected adult female Ixodes scapularis. Xenodiagnosis revealed that all 11 nonvaccinated control dogs and 2 of 10 vaccinated dogs were infected with B. burgdorferi. Six of 11 control dogs also developed fever (0.75 +/- 0.38 degrees C) and were lethargic. One of the control dogs also developed a limp. Both of the infected vaccinated dogs were asymptomatic. Thus, the vaccine prevented tick-vectored infection and associated symptoms of Lyme disease.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adjuvants, Immunologic*
  • Animals
  • Antibodies, Bacterial / biosynthesis
  • Antibodies, Bacterial / blood
  • Antigens, Bacterial*
  • Antigens, Surface / immunology*
  • Bacterial Outer Membrane Proteins / immunology*
  • Bacterial Vaccines* / immunology
  • Borrelia burgdorferi Group / immunology*
  • Borrelia burgdorferi Group / isolation & purification
  • Dogs
  • Female
  • Immunoglobulin G / biosynthesis
  • Immunoglobulin G / blood
  • Lipoproteins*
  • Lyme Disease / prevention & control*
  • Male
  • Saponins / immunology*
  • Ticks / microbiology
  • Vaccination
  • Vaccines, Synthetic / immunology

Substances

  • Adjuvants, Immunologic
  • Antibodies, Bacterial
  • Antigens, Bacterial
  • Antigens, Surface
  • Bacterial Outer Membrane Proteins
  • Bacterial Vaccines
  • Immunoglobulin G
  • Lipoproteins
  • OspA protein
  • Saponins
  • Vaccines, Synthetic
  • OspB protein, Borrelia burgdorferi
  • saponin QA-21V1