Multi-slice MRI of rat brain perfusion during amphetamine stimulation using arterial spin labeling

Magn Reson Med. 1995 Feb;33(2):209-14. doi: 10.1002/mrm.1910330210.


When a single coil is used to measure perfusion by arterial spin labeling, saturation of macromolecular protons occurs during the labeling period. Induced magnetization transfer contrast (MTC) effects decrease tissue water signal intensity, reducing the sensitivity of the technique. In addition, MTC effects must be properly accounted for in acquiring a control image. This forces the image to a single slice centered between the labeling plane and the control plane. In this work, a two-coil system is presented as a way to avoid saturation of macromolecular spins during arterial spin labeling. The system consists of one small surface coil for labeling the arterial water spins, and a head coil for MRI, actively decoupled from the labeling coil by using PIN diodes. It is shown that no signal loss occurs due to MTC effects when the two-coil system is used for MRI of rat brain perfusion, enabling three-dimensional perfusion imaging. Using the two-coil system, a multi-slice MRI sequence was used to study the regional effects of amphetamine on brain perfusion. Amphetamine causes significant increases in perfusion in many areas of the brain including the cortex, cingulate, and caudate putamen, in agreement with previous results using deoxyglucose uptake to monitor brain activation.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amphetamine / pharmacology*
  • Animals
  • Arteries
  • Body Water
  • Brain / blood supply*
  • Brain / drug effects*
  • Brain / pathology
  • Cerebral Cortex / blood supply
  • Cerebral Cortex / drug effects
  • Cerebral Cortex / pathology
  • Cerebrovascular Circulation / drug effects*
  • Cerebrovascular Circulation / physiology*
  • Gyrus Cinguli / blood supply
  • Gyrus Cinguli / drug effects
  • Gyrus Cinguli / pathology
  • Hypercapnia / physiopathology
  • Image Enhancement / instrumentation
  • Image Enhancement / methods
  • Macromolecular Substances
  • Magnetic Resonance Imaging / instrumentation
  • Magnetic Resonance Imaging / methods*
  • Male
  • Olfactory Pathways / blood supply
  • Olfactory Pathways / drug effects
  • Olfactory Pathways / pathology
  • Putamen / blood supply
  • Putamen / drug effects
  • Putamen / pathology
  • Rats
  • Rats, Sprague-Dawley
  • Spin Labels


  • Macromolecular Substances
  • Spin Labels
  • Amphetamine