Biopolymer synthesis on polypropylene supports: oligonucleotide arrays

Anal Biochem. 1995 Jan 1;224(1):110-6. doi: 10.1006/abio.1995.1014.


High-density oligonucleotide arrays have been constructed on clear, aminated polypropylene film using conventional phosphoramidite-based synthesis chemistries. A semiautomated 64-channel fluidic chemical delivery system is used to prepare the 64 x 64 array containing 4096 oligonucleotide elements. The completed array has been successfully used in the hybridization and fluorescence (Streptavidin-FITC) detection of a biotinylated 18-mer oligonucleotide target complementary to the F508 codon region of the cystic fibrosis transmembrane conductance regulator gene. A hybridization simulation model based upon Nearest Neighbor approximations is used to predict the outcome of the array hybridization experiment.

MeSH terms

  • Base Sequence
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Oligonucleotides / chemical synthesis*
  • Polypropylenes


  • Oligonucleotides
  • Polypropylenes