A pathway for biodegradation of 1-naphthoic acid by Pseudomonas maltophilia CSV89

Arch Microbiol. 1995 Jan;163(1):42-7. doi: 10.1007/BF00262202.


Pseudomonas maltophilia CSV89, a bacterium isolated from soil in our laboratory, grows on 1-naphthoic acid as the sole source of carbon and energy. To elucidate the pathway for degradation of 1-naphthoic acid, the metabolites were isolated from spent medium, purified by TLC, and characterized by gas chromatography-mass spectrometry. The involvement of various metabolites as intermediates in the pathway was established by demonstrating relevant enzyme activities in cell-free extracts, oxygen uptake and transformation of metabolites by the whole cells. The results obtained from such studies suggest that the degradation of 1-naphthoic acid is initiated by double hydroxylation of the aromatic ring adjacent to the one bearing the carboxyl group, resulting in the formation of 1,2-dihydroxy-8-carboxynaphthalene. The resultant diol was oxidized via 3-formyl salicylate, 2-hydroxyisophthalate, salicylate and catechol to TCA cycle intermediates.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biodegradation, Environmental
  • Carboxylic Acids / metabolism*
  • Cell-Free System / metabolism
  • Chromatography, Thin Layer
  • Culture Media
  • Gas Chromatography-Mass Spectrometry
  • Hydroxylation
  • Naphthalenes / metabolism*
  • Oxidation-Reduction
  • Oxygen Consumption
  • Pseudomonas / enzymology
  • Pseudomonas / growth & development
  • Pseudomonas / metabolism*


  • Carboxylic Acids
  • Culture Media
  • Naphthalenes
  • 1-naphthoic acid