Elevated levels of members of the STAT family of transcription factors in breast carcinoma nuclear extracts

Br J Cancer. 1995 Apr;71(4):840-4. doi: 10.1038/bjc.1995.162.

Abstract

The transcription factor, milk protein binding factor (MPBF/Stat5), is a member of the STAT family of signalling molecules which mediates prolactin signal transduction in lactating mammary gland by binding to GAS (gamma-interferon activation site) DNA elements. We have determined the levels of STAT factors in nuclear extracts from a variety of human breast tissues including carcinoma and normal 'resting' breast by electrophoretic mobility-shift assay. The results show that the level of STAT binding activity is low in normal 'resting' breast and benign lesions while carcinoma samples have significantly higher (P < 0.01) amounts of STAT binding activity. Supershift analysis suggests that Stat1 and possibly other members of the STAT family of signalling factors, including Stat3, are activated in breast cancer tissues.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenofibroma / metabolism
  • Adenofibroma / surgery
  • Base Sequence
  • Binding Sites
  • Breast / pathology
  • Breast Diseases / metabolism*
  • Breast Diseases / surgery
  • Breast Neoplasms / metabolism*
  • Breast Neoplasms / surgery
  • Carcinoma in Situ / metabolism
  • Carcinoma in Situ / surgery
  • Cell Nucleus / metabolism*
  • DNA-Binding Proteins / isolation & purification
  • DNA-Binding Proteins / metabolism*
  • Female
  • Humans
  • Hyperplasia
  • Milk Proteins*
  • Molecular Sequence Data
  • Oligodeoxyribonucleotides
  • STAT5 Transcription Factor
  • Trans-Activators / isolation & purification
  • Trans-Activators / metabolism*
  • Transcription Factors / metabolism*

Substances

  • DNA-Binding Proteins
  • Milk Proteins
  • Oligodeoxyribonucleotides
  • STAT5 Transcription Factor
  • Trans-Activators
  • Transcription Factors