Kidney drug metabolizing activities in streptozotocin diabetic rats

Gen Pharmacol. 1995 Jan;26(1):137-41. doi: 10.1016/0306-3623(94)00163-h.

Abstract

1. Streptozotocin-induced diabetes produced significant changes on the drug metabolizing enzyme machinery of rat kidney microsomes. 2. It reduced the cytochrome P-450 content by 30%, this effect being reversed by insulin therapy. 3. Total androstenedione oxidative metabolism was increased 2.5-fold and insulin treatment partially antagonized this activation. 4. Total testosterone hydroxylase activities were not modified by diabetes nor by insulin but the formation of 2 alpha OH testosterone and 6 beta OH testosterone were distinct in diabetes or insulin treated diabetic rats. 5. Only UDP-glucuronyltransferase activity for PNP was found in kidney microsomes. Diabetes determined a lower UDPGT substrate efficiency not reversed by insulin therapy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Androstenedione / metabolism
  • Animals
  • Cytochrome P-450 Enzyme System / physiology
  • Diabetes Mellitus, Experimental / metabolism*
  • Glucuronosyltransferase / metabolism
  • Kidney / metabolism*
  • Male
  • Rats
  • Rats, Wistar
  • Streptozocin
  • Testosterone / metabolism

Substances

  • Testosterone
  • Androstenedione
  • Streptozocin
  • Cytochrome P-450 Enzyme System
  • Glucuronosyltransferase