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, 180 (2), 273-80

Cassette Vectors Directing Expression of T Cell Receptor Genes in Transgenic Mice

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Cassette Vectors Directing Expression of T Cell Receptor Genes in Transgenic Mice

V Kouskoff et al. J Immunol Methods.

Abstract

We describe a pair of cassette vectors that can be used to express rearranged T cell receptor genes in transgenic mice. Short DNA fragments containing rearranged V alpha and V beta segments are readily amplified from T cells and introduced between artificial cloning sites. Transgene-derived mRNAs are transcribed under the control of the natural TCR alpha and -beta promoter/enhancer elements. Using this vector, we have obtained transgenic mouse lines which display transgene-encoded TCR alpha and beta chains on a majority of T cells.

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