Cassette Vectors Directing Expression of T Cell Receptor Genes in Transgenic Mice

J Immunol Methods. 1995 Mar 27;180(2):273-80. doi: 10.1016/0022-1759(95)00002-r.

Abstract

We describe a pair of cassette vectors that can be used to express rearranged T cell receptor genes in transgenic mice. Short DNA fragments containing rearranged V alpha and V beta segments are readily amplified from T cells and introduced between artificial cloning sites. Transgene-derived mRNAs are transcribed under the control of the natural TCR alpha and -beta promoter/enhancer elements. Using this vector, we have obtained transgenic mouse lines which display transgene-encoded TCR alpha and beta chains on a majority of T cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Gene Expression Regulation / genetics*
  • Genetic Vectors / genetics*
  • Mice
  • Mice, Transgenic
  • Molecular Sequence Data
  • Mutagenesis, Insertional
  • Receptors, Antigen, T-Cell / biosynthesis*
  • Receptors, Antigen, T-Cell / genetics*

Substances

  • Receptors, Antigen, T-Cell